The selective agglutination of immunosensitized latex microspheres in the presence of the opposite analytes leads to frequency changes recognized by the QCM monitor (7-9).
The specific agglutination of SjAg-derivatized Si[O.
1/2] change of the assay solution or, conversely, steric hindrance of silica agglutination that is too high, as manifested by frequency responses.
Because composition of the assay buffer may play a vital role in controlling the physicochemical processes of immunological recognition and agglutination (13,24-25), we sought to optimize the composition of the reaction medium for the immunoagglutination assays within required ranges of pH, ionic strength, and surfactants.
Such a neutral condition might optimize the recognition of SjAb by SjAg-sensitized silica particles and subsequently the agglutination by appropriate electrostatic forces, because the immunoagglutination interaction process might involve pH-dependent charged protein species.