Ion exchangers on agarose
: cross-linked carriers diethylaminoethyl agarose
(anion exchanger) and carboxymethyl agarose
DNA fragments were separated and analyzed by agarose
Nucleic acid separation techniques include: self made agarose
or polyacrylamide gels, precast agarose
or polyacrylamide gels, capillary electrophoresis, or lab-on-chip microfluidic methods.
The cast agarose
gel is placed in a tank full of the same buffer used to melt the agarose
(this is done to avoid osmotic gradients between the gel and the buffer, which would interfere with what we're about to do).
visualize the PCR products in a horizontal agarose
The microsponges are 280-micrometer beads of agarose
, a cheap, common, lab-friendly material derived from seaweed and often used as a matrix for growing live cells or capturing proteins.
forms a type of physical gel, with the junctions arising from a phase transition.
In all cloning experiments, the vector was cut by the appropriate restriction enzyme and run on a 1% agarose
PCR products were analyzed by agarose
gel electrophoresis, and the expected 1,041-bp amplicon was excised from the gel.
As expected, in all 4 cases, abnormal lipoproteins such as lipoprotein-X (Lp-X) and Lp-Y were detected by agarose
gel electrophoresis (1 [micro]L of serum subjected to electrophoresis on agarose
gel at 90 V for 46 min; gel then stained with Fat Red 7B or stained for cholesterol and TGs), suggesting that differential reactivity to these abnormal lipoproteins may have caused the difference between the 2 assays.
The entire sample volume was then diluted to a total protein concentration of 1 mg/mL in phosphate-buffered saline and incubated with 35 [micro]L of a 50% slurry of protein A agarose
beads (Pierce, Rockford, IL) in phosphate-buffered saline and rocked at 4[degrees]C for 10 min to preclear the samples of endogenous immunoglobulin.
DNA from these colonies was extracted and run on agarose
Passage in reverse order over dodecyl agarose
removes nearly all of the remaining protein, but the FRED elutes almost unretarded.
He then used the molds to create leaf-surface replicas made of agarose
Quotations are invited for Purchase Of Chemicals And Reagents (Make: Himedia) :Rnase Solution , 100Bp Dna Ladder (50 Lanes) ,100L, Glutaraldehyde Solution, 25%, Agarose
Special, Low Eeo, A Ready To Use Mixture Of Dntps, Lysozyme, Tris Base, Proteinase K, 6X Orange Gel Loading Buffer, Water, Sterile, Molecular Biology Grade, Oroacetic Acid, Hi-Artm/Acs, Taq Polymerase (5U/L) Opening date : 28 Dec 2017
Purchase Of Chemicals And Reagents (make: Himedia) :rnase Solution , 100bp Dna Ladder (50 Lanes) ,100l, Glutaraldehyde Solution, 25%, Agarose Special, Low Eeo, A Ready To Use Mixture Of Dntps, Lysozyme, Tris Base, Proteinase K, 6x Orange Gel Loading Buff