They devised a method of stretching a normally floppy actin filament
taut between two laser beams and then lowering the filament to touch a single myosin molecule, Spudich explains.
Cells treated for 24 hr with 10-6 M FL (Figure 2B), however, contained no visible stress fibers, but rather exhibited shortened and disorganized actin filaments
2+] sensitive, 41,000-dalton protein which reversibly blocks the "barbed" ends of actin filaments
but does not sever them.
Palladin is a regulator of actin filament
bundles at the ectoplasmic specialization in adult rat testes," Endocrinology 154(5): 1907-1920.
The fission yeast cytokinesis formin Cdc12p is a barbed end actin filament
capping protein gated by profilin.
Secretory granules bound to the end of an actin filament
can move to the plasma membrane as a result of the contraction of acto-myosin that is activated by [Ca.
Underlying the activity of muscle contraction is an actin filament
sliding on myosin molecules in the presence of adenosine triphosphate (ATP) to be hydrolyzed.
Cytochalasin B slows but does not prevent monomer addition at the barbed end of the actin filament
The viruses assemble these tails from intracellular actin filaments
Then, in the March 17 NATURE, a group of scientists reported seeing the squiggling motion of actin filaments
The mutation (called R156W), was first identified in an individual suffering from cochlear hearing loss, and it affects the way the myo1c protein interacts with proteins known as actin filaments
, another crucial component of the sensory apparatus of the inner ear.
David Williams, who earned his doctorate at the UW while conducting the research, said that the predominant thinking of the last 50 years is that 100 percent of the muscle force comes from changes as muscles shorten and myosin and actin filaments
To address this growing need, GeneTex has developed a new line of subcellular and organelle marker antibodies that are highly specific to ribosomes, centrosomes, mitochondria (inner and outer membrane), the endoplasmic reticulum, the Golgi apparatus, kinetochores, lysosomes, microtubules/spindles, actin filaments
, Cajal bodies, the nuclear envelope, the nucleolus and the nucleus.
Using a technique to make a pattern of active myosin molecules on a surface, they showed how cargo -- they used small beads -- could be attached to actin filaments
and moved from one part of the surface to another.
Another mechanism leading to cholestasis is mediated by drugs that bind to actin filaments
, resulting in disruption of the actin filaments
situated around the bile canaliculi, thus preventing the normal pulsatile contractions that move bile through the canalicular system to the bile ducts (Cullen 2005).