Analyzed sequences of the 5' and 3' termini and restriction sites were identical to the wild-type virus
Using this approach, a CSF virus was genetically manipulated not only to attenuate it, but also to introduce a genetic marker that can distinguish it from wild-type virus
As a result of mutations in the stems of the V3 loop, resistant virus binds with higher affinity to the drug-bound co-receptor than wild-type virus
A second recombinant vector is prepared with RT-PR sequences from the wild-type virus
is virus as it comes out of nature, i.
The pediatricians deemed that rate too high now that the wild-type virus
has been eradicated in this country, says Sutter.
Other suspected cases that have been reported turned out, upon polymerase chain reaction testing, to involve wild-type virus
in the contact's rash, indicating that these weren't secondary transmissions after all.
Will the genetically modified virus vector spread and persist as well as the wild-type virus
, and so on?
When they began their new regimens, 19 of those 24 saw the virus in their blood return to undetectable levels, compared with just one of nine people who did not experience a reversion to the wild-type virus
Cell culture isolation of virus is not a useful approach to diagnosis, however, because wild-type virus
usually replicates poorly in cell culture.
After five of these competition passages, the final ratio of Fpop40 to wild-type virus
was determined by plating as described above.
In this study, we developed a single-nucleotide polymorphism (SNP) real-time reverse transcription PCR (RT-PCR) to differentiate NA 292K mutant virus from R292 wild-type virus
in clinical samples.
We found that when the new vaccine, containing gene sequences similar to the wild-type virus
, was used in vaccination studies, the vaccinated birds were protected from disease and shed less of the wild-type virus
after challenge," says Yu.
The wild-type virus
is found in the natural infection, in contrast to the virus strain found in vaccine.
Virological failures were 11 (7%) versus 5 (3%) with evidence of one case of wild-type virus
in both arms and one subject with an emergent PI resistance at failure (10I/V, 48V, 54V, 82A, 84V) in the SQV/r arm.