Vascular remodeling and arterial calcification are directly mediated by S100A12 (EN-RAGE) in chronic kidney disease.
S100A12 expression in thoracic aortic aneurysm is associated with increased risk of dissection and perioperative complications.
In the absence of ELISAs specific for the monomeric forms of S100A8 and S100A9 and of a commercially available test for S100A12
, we have hypothesized that mass spectrometry (MS) might be a possible approach for detecting these proteins (22).
High levels of S100A12
are associated with recent plaque symptomatology in patients with carotid atherosclerosis.
induces the expression of endothelial adhesion molecules, promoting leukocyte recruitment (33), neutrophil oxidative burst, degranulation and secretion (34), and pulmonary mast cell activation (35).
sup] Using immunohistochemical analysis, S100A8, S100A9, S100A12
, and S100[sz] levels were shown to be associated with vascular structures in brains.
In a first step we cloned human S100B, S100Al through -A6, S100A12
, and S100A13 into expression vectors for high expression in Escherichia coli and purified the recombinant proteins by ammonium sulfate precipitation, followed by [Ca.
, the pro-inflammatory RAGE-ligand, elevated in CKD 5 stage patients and is an independent predictor of mortality risk.