3a) While enzyme HinfI showed 35 DNA restriction fragment
(Table-3) and allowed four unique species specific restriction profiles and HaeIII enzyme obtained 40 DNA restriction fragments
(Table-3) of diazotrophic isolates indicating four unique species specific restriction pattern as obtained within the genus (Fig.
Because a large fraction of the intestinal community is difficult or not posible to culture, molecular approaches such as Restriction Fragment
Length Polymorphism (RFLP), has been shown to be an useful tool for the study of diet-associated changes in gut microbial communities.
The amplified restriction fragment
length polymorphism (AFLP) technique is a relatively new genotypic method that has not yet been widely used in parasitology (13).
Size of restriction fragment
(bp) Isolates Rsa I Alu I Hha I Eco RI M1 and M2 NC NC 270, 185, 125 320, 290 M3 to M6 390, 190 365, 175 270, 185, 145 290, 330 M7 NC 385, 200 270, 185, 155 320, 300 M8 to M30 390, 190 365, 175 270, 185, 145 290, 330 M31 to M37 NC NC 270, 185, 125 320, 290 M38 to M40 NC NC 270, 185, 145 320, 290 G1 to G10 380, 180 365, 175 270, 185, 145 290, 300 C1 380, 180 360, 195 270, 185, 145 290, 330 C2 500 NC 185, 280, 135 315, 295 Table 2--Restriction fragments
(bases of pairs-bp) of the endonucleases used in rDNA internal transcribed spacer (ITS) amplified of Colletotrichum spp.
Similar to macrorestriction mapping, it provides lengths for long restriction fragments
Different molecular methods for the identification of rarely isolated nontuberculous mycobacteria and description of new hsp65 restriction fragment
length polymorphism patterns.
Gene-Mapper software (Applied Biosystems Instruments, Foster City, California) was used to measure terminal restriction fragments
up to 600 bp in length with peak heights above 200 fluorescent units.
A total of 24 specimens were identified to species or species group based on restriction fragment
patterns of the mtDNA using one or more restriction endonucleases (Table 3) and without knowledge of the morphological assignment.
These enzymes have little practical research value as they do not produce coherent restriction fragments
The double intensity restriction fragments
suggest the presence of duplicated chromosome segments.
Genomic diversity was studied by pulsed-field gel electrophoresis (PFGE) and analysis of XbaI restriction fragments
as described previously (3).
Ehrat, Integrated capillary electrophoresis on flexible silicone microdevices: Analysis of DNA restriction fragments
and detection of single DNA molecules on microchips, Anal.
The PCR products were digested for 1 h at 37 [degrees] C, and the restriction fragments
were run out on a 1.
The size of the chromosome of Psm was the result of the summation of the estimated size of individual DNA restriction fragments
generated with PacI, PmeI and Swal and separated by PFGE.
were separated by gel electrophoresis through 2 per cent TBE agarose gel (Genei, Bangalore).