Variation in restriction fragment
length polymorphism could be seen.
The amplified restriction fragment
length polymorphism (AFLP) technique is a relatively new genotypic method that has not yet been widely used in parasitology (13).
Similar to macrorestriction mapping, it provides lengths for long restriction fragments
Gene-Mapper software (Applied Biosystems Instruments, Foster City, California) was used to measure terminal restriction fragments
up to 600 bp in length with peak heights above 200 fluorescent units.
Restriction enzymes seek a specific sequence, typically between four and eight bases in length, and cut the DNA at this location, creating discrete restriction fragments
were separated by gel electrophoresis through 2 per cent TBE agarose gel (Genei, Bangalore).
After samples were incubated at 37[degrees]C for 60 min, the restriction fragments
were separated by agarose gel electrophoresis.
length polymorphism analysis of Cryptococcus neoformans isolates from environmental (pigeons excreta) and clinical sources in New York City.
Following the digestion, two restriction fragments
of 650 and 175 bp were observed for GG homozygotes, a single 825 bp band was obtained for AA homozygotes, and AG individuals displaying all three bands.
The significant differences obtained in dynamic range did not depend on which spectral band was chosen for quantification among the 10 restriction fragments
, except for a pair of 7mers coming from the H3 motif.
were separated by PFGE as described (5), transferred to nylon membranes, and hybridized with the probe.
3) Does intraspecific variability compromise the use of mtDNA restriction fragments
in species identification?
The restriction fragments
were separated by a 1 per cent agarose gel electrophoresis run in 1x TBE (Tris-borate EDTA) overnight at 1.
PCR restriction fragments
were separated by electrophoresis in 2% Response Research (Biozym) agarose gels (Fig.
Insertions, duplications, inversions, and deletions can cause changes in restriction fragments