Molecular epidemiology of Shigella infections: plasmid profiles, serotype correlation, and restriction endonuclease
patent 8,921,332 issued on December 30th, 2014, which claims the use of chimeric restriction endonucleases
for directing chromosomal gene editing in cells by homologous recombination.
To construct the physical map of the chromosome of Psm, three rare-cutting restriction endonucleases
were chosen based on G+C content of P syringae strains, several enzymes with A+T rich recognition sequences were tested, among these enzymes PacI (TTAATTAA), PmeI (GTTTAAAC) and SwaI (ATTTAAAT) were selected which originated 14, 15 and 16 fragments, respectively.
Plasmid DNA was isolated from an overnight bacterial culture and cut with restriction endonucleases
which found on the map of cloning vector.
At 10 and 24 hpf, gDNA extracted from TDCPP-treated embryos was incompletely digested by all methylation-sensitive restriction endonucleases
tested, and banding patterns were similar to those of vehicle-treated controls (Figure 6).
A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease
mapping and DNA sequence data.
The mtCOI fragment (about 620bp) was first cleaved by the restriction endonucleases
VspI (Khasdan et al.
Mathematical model for studying genetic variation in terms of restriction endonucleases
A variation of fragments from 90 to 500bp was obtained after digestion with the restriction endonucleases
Rsa I, Alu I, HinfI, Hpa II, Hha I, Xho I, Acc I, Eco RI and Taq I (Tables 1 and 2).
And perhaps the most telling example of all--the discovery of restriction endonucleases
, which enabled the whole field of synthetic molecular biology and biotechnology.
Potentially useful restriction endonucleases
identified by the virtual digestion analyses were then tested in a PCR/ RFLP agarose gel analysis of the ITS-1 marker in multiple individuals of each species.
For over ten years, the vast majority of molecular methods that analysed variation in DNA sequence relied on the special properties of the second major discovery, restriction endonucleases
Use of low-frequency-clevage restriction endonucleases
for DNA analysis in epidemiological investigations of nosocomial bacterial infections.
In this case, methylation analysis involving the use of methylation-sensitive restriction endonucleases
could be used, thus cutting hypomethylated RASSF1A from maternal blood cells and leaving the hypommethylated placental RASSF1A intact for amplification.
Polysaccharide contaminations are particularly problematic as they inhibit the activity of many commonly used molecular biological enzymes, such as polymerases, ligases and restriction endonucleases