For application to the field of methylation, however, this restriction should not be an issue, because bisulfite treatment removes the cytosines from PspGI recognition sequences
and prevents illegitimate digestion of target sequences.
Two nested sets of universal primers flanking the variable regions, CV160F/CV100R and CV140F/CV120R, were selected and modified to introduce a type IIS restriction endonuclease recognition sequence
ahead of the variable regions on amplification.
A sodium iodide symporter is genetically fused to either the N-terminus or C-terminus of the product of a transgene through a linker peptide which bears the recognition sequence
of a host cell protease.
Cytovia's services center around its proprietary technology for high-throughput screening in living cells, which is applicable to any protease for which the substrate recognition sequence
The patent covers methods and compositions, which facilitate the isolation and specific targeting of any single stranded DNA sequence to be acted upon by any desired double stranded DNA genetic element or recognition sequence
Only one of the two SNP alleles forms a full recognition sequence
together with the 3' SNP restriction site and will be cleaved.
This method depends on the fact that the mutation of interest disrupts a recognition sequence
for a restriction enzyme.