The root of Phytolaccaceae (Phytolacca americana var.
Two-year old Phytolaccaceae roots were harvested in November 1991, and the extracts of Cordyceps sinensis (CS) were kindly supplied from Dr.
At the final incubation period, to examine the effects of Phytolaccaceae extracts on high-ambient glucose-induced mesangial proliferation, cells were simultaneously treated with several different concentrations (2.
Cytotoxicity of Phytolaccaceae was determined by counting the number of cells that remained adherent to the cell monolayer and excluded trypan blue.
Inhibitory effect of Phytolaccaceae on high glucose-induced GMC proliferation was examined, and CS was simultaneously used as a positive control compound (Lin et al.
Cell viability was estimated to clarify whether inhibition of high glucose-induced GMC proliferation by Phytolaccaceae resulted from the cytotoxic effects of herbal extracts.
The stimulation was significantly blocked by treatment of GMCs with 10 [micro]g/ml of CS or Phytolaccaceae for 24 hrs (p < 0.
The effects of Phytolaccaceae on the synthesis of other ECM components were also studied by measuring fibronectin contents in the GMCs at the completion of the same experiments above, and the obtained result are shown in Fig.
5, TGF-[beta]1 secretion into media was significantly increased by exposure of GMCs under 20 mM glucose conditions, and this stimulation was significantly blocked by treatment of CS or Phytolaccaceae (10 [micro]g/ml).
The present study examined whether Phytolaccaceae extract impairs the ability of high glucose to accumulate ECM components and to induce cell proliferation in cultured GMCs.
Regarding these, this study investigated the effects of Phytolaccaceae extracts on high glucose induced-proliferation of GMCs in culture.
This stimulation was significantly inhibited by treatment of GMCs with Phytolaccaceae extracts.
Based on the pathological significance of TGF-[beta]1 in diabetic nephropathy, this study investigated the effects of Phytolaccaceae on high glucose-induced TGF-[beta]1 production in cultured GMCs, and obtained the results shown in Fig.