Peering into the genomes of both the bacteria and their pathogens, he discovered the first evidence of anti-CRISPR proteins, which help phages
slip past bacterial defenses.
coli O157:H7 occurred at 5 C in the presence of phage
FAHEc1 at greater than 107 plaque-forming units per ml in broth in vitro.
that infect clinically relevant strains of C.
Bacteria can "steal" small pieces of DNA from phages
that attack them, and use these stolen pieces to recognize and respond to the attacker, in a manner similar to usage of antibodies by the human immune system.
Scientists of the day did not understand exactly how phages
killed bacteria, and their crude therapies performed inconsistently.
Two different attachment sites were found for this family of phages
on the V.
[PHI] SH-56 had an icosahedral head, about 70 nm in diameter, and a 100-nm long tail, thus morphologically similar to phages
belonging to Siphoviridae family
reproduce by insinuating themselves into bacteria.
He has also tested the phages
on their prime target: refrigerated fresh-cut lettuce.
After the discovery of bacteriophages in the early 1900s, researchers sought phages
that would target and destroy infectious bacteria.
, which are viruses that infect bacteria, cut through plaques in the brains of mice engineered to develop a disease similar to Alzheimer's.
are naturally occurring agents that target and destroy bacteria with a high degree of efficiency, and do so selectively and specifically, without affecting beneficial bacteria or body cells.
The value of the new patent is that it creates the opportunity for phages
to be used as a systemic therapeutic, that is, potentially as an injectable, in both human and veterinary medicine.
The scheme consists of 11 phages
and is based on the typing of the rough phase II variant of S.
were generated according to the manufacturer's instructions with minor modifications.