Prevention of angiogenesis by naked DNA
IL-12 gene transfer: angioprevention by immunogene therapy.
It was indicated that co-delivery of naked DNA
E7+gp96 plasmid was immunologically more effective than E7 DNA and it could be an effective approach to induce E7-specific immune responses as a potential vaccine candidate for cervical cancer (55).
Lipid spheres can carry DNA, or naked DNA
can be injected directly into muscles.
Cross linked plasmid DNA-gelatin nanospheres containing about 25-30% DNA were found to be more resistant to nuclease digestion than naked DNA
and induced greater gene expression in mice after intramuscular injection (17).
In several cases naked DNA
was employed successfully, but DNA-Liposome complexes or electroporation procedures have been also used (Spadafora).
As far as we know, naked DNA
poses none of the risks associated with viral vectors.
But direct injection of naked DNA
just doesn't work," she notes.
The first method used a catheter system to inject naked DNA
for vascular endothelial growth factor directly into the myocardium of the left ventricle with a special catheter system.
Of course, the excretion of naked DNA
and DNA from dying cells can be different.
Q: Are you using naked DNA
to transport the new genes into the body, like that used at St.
Lee testified, "The naked DNA
in the vaccine was probably stabilized through a chemical binding between the mineral aluminum and the phosphate backbone of the double-stranded DNA.
Vaccines currently under development include subunit vaccines (9), naked DNA
vaccines (10,11), and attenuated mycobacteria, including recombinant BCGs expressing immunodominant antigens and cytokines (12).
For 2 decades, away from the noise of the latest ups and downs for viral vectors, chemists and materials scientists have been doggedly investigating and improving on other strategies such as using capsules that protect and guide DNA into cells and methods of introducing naked DNA
The naked DNA
or the adenoviruses are simply a means of getting any kind of gene expressed.
A: Naked DNA
is simply a means of getting any kind of gene into the body.