4%) of the 51 tested, apparent false positive isolates yielded a single colony type on MacConkey agar
, produced magenta colonies on mTEC agar, and were identified as E.
APAS achieved an overall sensitivity of more than 96% in its ability to combine the results from blood agar and MacConkey agar
in assessing the presence of bacteria commonly associated with UTIs.
2) Nonstandard abbreviations: STEC, shiga toxin-producing Escherichia coli; SMAC, MacConkey agar
The observation of growth on sheep blood agar and MacConkey agar
is an important first step.
Microbiology specialist Lab M, has added a modified MacConkey Agar
to its range of dehydrated culture media.
This study cultured specifically for facultative anaerobic mesophilic bacteria, aerobic mesophilic bacteria, and thermophilic bacteria on BHI; enteric bacteria were cultured on brilliant green agar (BGA) and MacConkey agar
A bacteriological loop was dipped in vial containing the mother sample and a loop full was streaked on MacConkey agar
(Oxoid) and is incubated at 37[degrees]C for 18-24 hours.
Samples for aerobic culture were plated on Columbia blood agar and MacConkey agar
(Remel, Lenexa, KS, USA) and incubated a minimum of 48 hours at 37[degrees]C with 5% carbon dioxide.
To estimate the array of bacteria obtained on the Mannitol salt and MacConkey agar
plates used in the sampling of the environmental surfaces, a subsample of colonies from 20 plates was examined using Gram-staining and the test described above for S.
In the Compared Pathology Laboratory for Wild Animals (Lapcom) of the Department of Pathology of the Faculty of Veterinary Medicine and Animal Science (FMVZ), University of São Paulo (USP), São Paulo State, Brazil, the organ was opened in a sterile manner and swabs of the mucosa were seeded in MacConkey Agar
(Difco[R]) and XLT4 Agar (Difco[R]) and kept in microbiological oven at 37[grados]C under aerobiosis for 24 hours.
100 [micro]l of the diluted sample was plated on STAA agar, Plate Count Agar, Sorbitol MacConkey agar
, Violet Red Bile Glucose agar, APT agar and the PS agar.
A sample provided on August 22, 2013, was confirmed positive for STEC by culturing an STEC strain on MacConkey agar
(bioMerieux, Marcy l'Etoile, France) that did not grow on selective agar (CHROMagar STEC, Mast Diag nostika, Reinfeld, Germany) optimized for the detection of classical enterohemorrhagic E.
The culture media used included nutrient agar, MacConkey agar
and chocolate agar.
Viable counts of bacteria in the fecal samples were then conducted by plating serial 10-fold dilutions (in 10 g/L peptone solution) onto MacConkey agar
plates (Difco Laboratories, Detroit, MI, USA) and lactobacilli medium III agar plates (Medium 638, DSMZ, Braunschweig, Germany) to isolate the E.
For cerebrospinal fluid (CSF), body fluids and respiratory specimens (sputum and bronchoalveolar lavage, BAL), sample was inoculated on sheep blood agar and MacConkey agar
for 48 h and incubated aerobically and in 5-10 per cent C[O.