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BMMs were seeded into Osteo assay plates and cultured with M-CSF and RANKL for 3 days until multinudeated osteoclasts were formed, and then they were treated with the R.
Las condiciones basales minimas para la formacion de los osteoclastos son la presencia de RANKL y M-CSF, que son producidos por los osteoblastos maduros, las celulas precursoras de la medula osea y las celulas T activadas.
Cells were cultured in [alpha]-MEM with 10% FBS supplemented with M-CSF (30 ng/mL), RANKL (100 ng/mL), 1% L-glutamine, 1% penicillin/streptomycin, and 1% nonessential amino acids (Gibco).
Dr Thornton said, 'The next step is to see whether maternal smoking can alter the level of growth factors, life M-CSF, which are produced by the placenta.
Recombinant human M-CSF was purchased from Kyowa Hakko Kogyo (Leukoprol; Tokyo, Japan).
Next, for cathepsin S, chemerin, interleukin (IL)-1RA, monocyte chemoattractant protein 1 (MCP-1), M-CSF, macrophage migration inhibitory factor (MIF), nerve growth factor (NGF), RBP-4, and SAA1, ELISAs with the same antibody pairs as the MIA were not available and were not performed.
Together with the activation of the receptor c-Fms by M-CSF, this will lead to an expansion of the osteoclast progenitor pool, increased survival of these cells, and the initiation of a differentiation program that terminates in fusion of the mononucleated progenitor cells and the development of latent multi-nucleated osteoclasts.
Table 2: World Market for Erythropoietins (2000-2010): Geographic Regions Ranked by CAGR - Europe, US, Japan and Rest of World (includes corresponding Graph/Chart) II-14 Colony Stimulating Factors II-14 Granulocyte-Colony Stimulating Factor II-14 Granulocyte Macrophage-Colony Stimulating Factor II-14 Other Colony Stimulating Factors II-14 Macrophage Colony Stimulating Factor (M-CSF) II-15 Key Functions of M-CSF II-15 Side-Effects of Colony-Stimulating Factors II-15