Sequence comparisons of the same PCR fragment among three pig populations detected one SNP (HQ026023:1383 G>C) in exon7 and two SNPs (HQ026023:130 G>A; 232 C>T) in the 5 promoter region of the porcine IRF6 gene.
Association analysis of porcine IRF6 gene in three pig populations
On the other hand, the results of association analysis between SNPs and immune traits also showed that the SNP (HQ026023:1383 G>C) of IRF6 gene was significantly associated with the level of IFN-[gamma] (d 20) in serum (p = 0.
IRF6 plays an important role in initiating cellular interferon-stimulated gene-mediated antiviral responses and in the transforming growth factor-p signaling pathway.
Our results provided a straightforward insight that the IRF6 gene which has effects on serum IFN-[gamma], IL-10 level and ratio of IFN-[gamma] to IL-10 in serum could served as a genetic marker for immune traits.
In summary, the 13,720 bp-length IRF6 genomic organization with seven exons and six introns was identified.