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Words related to hybridoma

a hybrid cell resulting from the fusion of a lymphocyte and a tumor cell

References in periodicals archive ?
Hybridomas that had optical density close to or above the estimated cut-off (OD = 0.
Then, hybridoma supernatant was added to the plates, and incubated for 12 h at 4[degrees]C.
32]P-labelled antisense V[alpha]14J[alpha]18 mRNA derived from one of the hybridomas was hybridized with total mRNAs (sense) from normal B6 thymocytes.
Immunochemical activity of the antibodies produced by the subclones did not differ from the values of the initial hybridomas.
By adding selective HAT medium, hybridoma cells were established and positive clones were selected by ELISA after four times of cloning.
MAbs recognizing FFL(Firefly luciferase) specificly were developed by obtaining hybridoma cell lines from the immunized animals injected with the firefly luciferase protein .
Therefore, the present study was aimed to establish an adaptation protCol for WM68 hybridoma cell line - from high to a low serum concentration and finally into a serum free culture condition.
1989, "Transient kinetic of hybridoma growth and monoclonal antibody production in serum limited cultures," Biotechnol.
Nevertheless, the need for facilitated screening of antibody-producing hybridomas in the late 1970s gave rise to Western blotting, again validating the Swiftian dictum of necessity leading to invention.
Hybridomas or other cell lines expressing such immunoglobulin molecules and monoclonal antibodies are also provided.
It is suitable for growing antibody-secreting cell lines, including hybridomas and Chinese hamster ovary (CHO) cells.
AutovaxID is suitable for growing antibody-secreting cell lines, including hybridomas and Chinese hamster ovary (CHO) cells which are currently the back bone of commercial therapeutic protein manufacture.
Hybridomas were selected in hypoxanthineaminopterin-thymidine medium and maintained in vitro by methods modified from Van Deusen (1983) in Dulbecco's modified Eagle's medium supplemented with 15% horse serum (Sigma).
The reactivity of the antibodies produced by the hybridomas was then determined.
The technology includes monoclonal antibodies and related hybridomas and other related know-how.