In previous observations of housekeeping gene
stability of B.
Many housekeeping genes
do show different expression patterns under different experimental conditions and the influence of physical exercise on housekeeping gene
expression is not well studied (Jemiolo and Trappe, 2004; Mahoney et al.
Caption: Figure 3: (a) Representative amplification curves of the quantitative real-time PCR for SOD2 and the housekeeping gene
beta-actin in hemodialysis patients (HD, circles), patients with chronic kidney disease (CKD, squares), and control subjects (healthy; triangles).
The relative p53-like gene expression was evaluated using 18S rRNA as a housekeeping gene
expression in bovine liver is affected by physiological state, feed intake, and dietary treatment.
ACTIN, a housekeeping gene
with expression unchanged during THH treatment, was chosen to serve as a control.
The [DELTA][DELTA]CT method was used to determine the fold change in mRNA expression relative to the housekeeping gene
, ribosomal protein, large, P0.
LUC] yields and housekeeping gene
ratios), we used the nonparametric paired Wilcoxon test.
jejuni housekeeping gene
, gltA, was used as a positive control.
RNA integrity of the cDNA preparations was tested by real-time PCR amplification of the porphobilinogen deaminase housekeeping gene
Both assays normalize target gene expression to a single housekeeping gene
Within a single array, the variability of housekeeping gene
expression was estimated to be between 8.
For each sample, the quality of DNA extraction was verified by real-time PCR for a housekeeping gene
encoding p-actin (7).
In all PCRs, a housekeeping gene
(GAPDH) was used to monitor genomic DNA contamination and equality of sample loading.
We investigated cDNA quality and quantity in all samples by measuring the mRNA expression of the housekeeping gene
cyclophilin A with the cyclophilin A TagMan[R] Endogenous Control reagent set (Applied Biosystems), according to the manufacturer's instructions.