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The mRNA expression level of H-FABP was measured using a 7500 Real-Time PCR System with a 20 [micro]L reaction system containing the following: 1 [micro]L of cDNA, 10 [micro]L of 2x SYBR Premix DimerEraser, 0.
Recently published research by the Manchester Acute Coronary Syndromes (MACS) group (Body, 2014) supports a protocol - the MACS Rules - that includes a combination of ECG, hsTn, and H-FABP results coupled with strongly indicative clinical symptoms such as persistent angina, radiating chest pain, sweating or vomiting.
The researchers examined H-FABP levels in the blood as well as troponin -- the biomarker traditionally used to diagnose a heart attack.
1 ng/mL) showed the largest variation in H-FABP levels, and the highest concentrations were observed for the SCI+PU subject (6.
2] Nonstandard abbreviations: AMI, acute myocardial infarction; cTnT, cardiac troponin T; Flt-1, fms-like tyrosine kinase; PlGF, placental growth factor; sFlt-1, soluble Flt-1; hs-cTnT, high-sensitivity cTnT (assay); NT-proBNP, N-terminal pro-B-type natriuretic peptide; H-FABP, heart-type fatty acid-binding protein.
Since little work has been done on A-FABP and H-FABP gene expression in chickens, the objective of this research was to detect the relative expression of A-FABP and H-FABP genes in different tissues of chickens using FQ-RT-PCR, and further analyze the correlation between transcription level of A-FABP and H-FABP genes and IMF content.
Recent trials have shown that H-FABP provides highly significant diagnostic value, especially during the early hours following ACS symptom onset.
In this study, amongst the many known genes associated with economic traits in pigs, the porcine GHRH, H-FABP and MYOG genes known to have a high association with growth or backfat thickness were selected.
One of the objectives of this study was to screen single nucleotide polymorphisms (SNP) of chicken H-FABP gene among 252 individuals that included 4 Chinese domestic chicken breeds (Fengkai Xinghua (T04), Huiyang Huxu (H), Qingyuan Ma (Q), Guangxi Xiayan (S1)), 2 breeds developed by the Institute of Animal Science, Guangdong Academy of Agricultural Sciences (Lingnan Huang (DC), dwarf chicken (E4)) and one introduced broiler (Abor Acre (AA)).
u] and H-FABP concentrations in circulation and in myocytes under physiologic and ischemic conditions.
How will troponin, CK-MB, and myoglobin tests fare once the pipeline biomarkers copeptin and H-FABP gain uptake?
The H-FABP content of skeletal muscle is only 10%-20% of that found in cardiac muscle, whereas the skeletal muscle content of myoglobin is approximately twice that of cardiac muscle (1-3).
Similar to the use of heart-type fatty acid-binding protein (H-FABP) as a plasma marker for the rapid detection of cardiac injury (13,14), brain-type FABP (BFABP) and H-FABP (15) may be suitable markers for the detection of brain injury.
The panel quantitatively tests whole blood specimens for levels of cardiac troponin I (cTnI), myoglobin and H-FABP, biomarkers often used as an aid in the diagnosis of myocardial infarction (MI).