Vitellogenesis occurred through a process of autosynthesis, involving the combined activity of the Golgi
complex, mitochondria and rough endoplasmic reticulum, and a process of heterosynthesis, in which extraovarian precursors were incorporated into oocytes by endocytotic activity at the basal region of the oolemma of the early vitellogenic oocytes.
Aunque recibio varias distinciones, como el premio Moscu (1900) y la medalla de oro Helmholtz de la Academia de Ciencias de Berlin (1905), el momento cumbre de su carrera fue en el mes de octubre de 1906, cuando el Real Instituto Carolino de Estocolmo le otorgo, junto con su colega Camillo Golgi
, el premio Nobel de Fisiologia y Medicina (1-4).
Si bien solo podia observar los axones de manera estatica, coloreados con una serie de tinciones argenticas, como el metodo de Golgi
y otras tecnicas clasicas de finales del siglo XIX, se quedo pensativo ante lo que veian sus ojos: ?
The cytoplasm contains numerous free ribosomes, large mitochondria, many vesicles and vacuoles containing electron-dense material; but the rough endoplasmic reticulum and Golgi
complexes are sparse (Figs.
sepioidea secretory cells showed differences in organelle abundance between the sexual maturity stages: at the juvenile and immature stages there are numerous mitochondriae, endoplasmic reticulum cisterns are thin and dispersed in the cytoplasm, the Golgi
apparatus has a curved profile and actively secretes granules of different densities, whilst in the IV maturation stage (females) and reproductive individuals (males) secretory cells are almost depleted of mitochondriae, endoplasmic reticulum cisterns are dilated and well developed in a concentric fashion, and the Golgi
complex actively secretes electron dense granules which are dispersed in the cytoplasm.
Even though we routinely observed Golgi
apparatus and rough endoplasmic reticulum (ER), we were unable to detect any specific labeling on these organelles.
tendon receptors play an important role in both of these techniques.
An Italian histologist, Camillo Golgi
(1843 or 1844-1926), fifteen years before, had devised a system of staining with silver compounds that brought out the structure of neurons in fine detail.
A eureka moment in Cajal's career occurred in Madrid in 1887 when another scientist showed him the Golgi
stain that made microscopic structures in brain tissue samples appear black against an amber background.
Glycosylation of proteins occurs in the endoplasmic reticulum and the Golgi
apparatus and is the most sophisticated post-translational modification of proteins.
Then, using cellular imaging, it was found that imidazopyrazines interfere with the function of PI4K on the parasite Golgi
(the organelle that packages proteins for delivery to other cellular destinations).
Several chapters describe the mechanism by which coat complexes facilitate vesicle budding from the endoplasmic reticulum and with retrograde traffic from the Golgi
back to the endoplasmic reticulum.
In human embryonic stem cells, the team found Bax standing at attention in its active form in the Golgi
apparatus, a part of the cell that processes and modifies proteins.
staining was used to visualize dendritic arborizations and spines, and routine Nissl staining was applied to visualize the somatic configuration of the PCs.
This method is an efficient way to identify N-glycosylation defects and to discriminate a genetic cause involving the cytoplasm or endoplasmic reticulum (CDG type I) from one involving the Golgi
apparatus (CDG type II).