B and C, Original slides were not retained, but representative cytology with modified Giemsa stain
shows caseous debris and granulomas and scattered Langhans giant cells, findings consistent with tuberculosis (original magnifications 310 [B] and 340 [C]).
In all animals, parasitaemia was determined on different days after infection using blood smears stained with Giemsa stain
(Sigma Chemical Co.
The 3 sections on the other slide were stained with Giemsa stain
Peripheral blood smears were made from venous blood samples collected in vacutainer tubes, containing ethylene diamine tetra-acetic acid (EDTA), and stained with Wright Giemsa stain
Petroleum ether, ethyl acetate, dimethyl sulphoxide, carbon dioxide, methanol, acetic acid, disodium hydrogen phosphate, monosodium dihydrogen phosphate, Wright's giemsa stain
, silica gel (60-120), Tris-HCl, ethylenediaminetetracetic acid (EDTA), sodium chloride (NaCl), sodium dodecylsulphate (SDS), ammonium acetate was purchased from Sisco Research Laboratory Pvt.
Slides were stained in 3% Giemsa stain
for 30 minutes, rinsed in tap-water and allowed to air-dry.
1) Sydney score/Atrophy (a) Negative/Negative, Giemsa stain
and ELISA serology both negative; Negative/Positive, Giemsa stain
negative and ELISA serology positive; Positive/Positive, Giemsa stain
and ELISA serology both positive.
Standard karyotypes were analyzed following staining with 2% giemsa stain
Sampling from patient lesions, staining tissue smears with Wright's Giemsa stain
and culturing parasites were performed based on the recent study (15).
The bright reddish-purple color of mucin with the modified Giemsa stain
is easy to recognize even on low magnification.
Microscopic examination showed double-walled spherical cysts in potassium hydroxide with calcofluor white stain, Gram stain (Figure, panels A, B), and Giemsa stain
Smears were fixed in methanol and stained with Giemsa stain