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a process used for separating mixtures by virtue of differences in absorbency

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Gel-filtration chromatography is a popular and versatile method that pursued the effective separation of proteins and high yield (O'Fagain et al.
Measurement of LDL particle size in whole plasma and serum by high-performance gel-filtration chromatography using a fluorescent lipid probe.
The final product gave a single peak on gel-filtration chromatography with a molecular weight of 70 kDa (Fig.
In contrast, measurements by high-performance gel-filtration chromatography and electrophoresic techniques such as GGE and tube gel electrophoresis probably reflect particle diameter more closely.
Comparison of LDL-C values for each fraction obtained by gel-filtration chromatography revealed that the New-Daiichi assay had a reactivity to Lp-Y of ~70% of that of the Denka-Seiken assay (Fig.
The 3 major forms of serum prolactin (PRL), identifiable by gel-filtration chromatography (GFC), are monomeric PRL (23 kDa), big PRL (45-60 kDa), and big, big PRL or macroprolactin (150-170 kDa) (1).
Conventionally, the diagnosis of macroprolactinemia has been based on the finding that more than 30-60% of prolactin was in the macroprolactin form as indicated by gel-filtration chromatography (12, 15, 23) or that <30% (23) or 40% (12,15) was recovered after treatment of the serum with polyethylene glycol (PEG).
It has been shown that the percentages of macroprolactin established by protein A precipitation and by gel-filtration chromatography, the reference method to identify macroprolactin, are well correlated (15).
Inspection of estimates of monomeric PRL by gel-filtration chromatography in these samples suggests that although the Bayer/Chiron PRL assays react with the macroprolactin present, these assays generally react to a lesser extent than others.
Heterogeneity of LDL particles with respect to size has been demonstrated by analytic ultracentrifugation (1), density-gradient ultracentrifugation, gradient-gel electrophoresis (GGE), high-performance gel-filtration chromatography, dynamic light scattering, and electron microscopy.