Protein convertases, notably furin
and PACE4, have been shown to play a role in tumor progression (61).
In the most critical position for furin
cleavage, P1, we found that >40% of FIPVs have a mutation in the arginine residue, which is replaced by an aliphatic (methionine and glycine) or polar uncharged (threonine) residue.
is a eucaryotic, subtilisin-like endoprotease that localizes to the trans-Golgi network of the constitutive secretory pathway and processes a wide variety of proproteins, including the precursors for several hormones and growth factors, receptors, blood proteins, bacterial toxins, and viral glycoproreins.
HEK 293 cells that normally express functional furin
(13,23) were transfected with the plasmids encoding proBNP-T71A or proBNP-WT and treated with a furin
inhibitor, Dec-RVKR-CMK, or cotransfected with a furin
Nakayama (27) produced a recombinant soluble form of furin
; to date, however, circulating furin
has not been identified.
Substrate cleavage analysis of furin
and related proprotein convertases: a comparative study.
3] Human genes: NPPB, natriuretic peptide B; NPPC, natriuretic peptide C; NPPA, natriuretic peptide A; FURIN
(paired basic amino acid cleaving enzyme).
JJ, Becerra MC, Shin SS, Kim JY, Bayona J, Farmer PE.
A plasmid expressing a well-established immune activator, granulocyte macrophage colony stimulating factor (GMCSF), and Gradalis' proprietary bifunctional short hairpin RNA construct against furin
is introduced into the cells by electroporation.
The ubiquitous endogenous convertase furin
or overexpressed corin did not result in accumulation of intracellular BNP32 (Fig.
Other men's favorites include Patrick Moulton the 2009 winner of the marathon and former New England Road Race Grand Prix Champion; Mohammed Awol, thirteenth place finisher in the New York Marathon; Lars Juhl, a second place finisher in the Champaign Marathon; and Mark Furin
, Stephen Haas, and Jeff Powers, members of Team Indiana Elite who are trying to qualify for the Olympic Trials; as well as Demesse Tefera the reigning 2009 National Half Marathon winner.
Becerra MC, Freeman J, Bayona J, Shin SS, Kim JY, Furin
JJ, et al.
Shin S, Furin
J, Bayona J, Mate K, Kim JY, Farmer P.
inhibitors also block proBNP processing, however, and a recent study reported that furin
and corin cleavage of human pro [BNP.
plays an extremely important role in mammalian physiology by endoproteolytically activating a number of growth factors, hormones, receptors, and blood proteins.