A combination of routine ultrastructural examination and freeze-fracture
analysis was used to show that the overall morphology of the UROtsa cells was most consistent with that found in the intermediate layers of in situ urothelium.
The reproduction of the original articles is excellent, including many transmission electron micrographs and the striking freeze-fracture
pictures of the cell membrane.
Jack Rosenbluth, a neurocytologist at New York University School of Medicine, is using a freeze-fracture
method to study nerve fibers in normal and in myelin-deficient animals.
analysis, confluent cultures of cells were subcultured onto mylar strips and fractured as described, previously (20).
or microtomed samples were immersed for 5 min in an agitated beaker containing chloroform at room temperature to remove the MPPO domains.