Although both isoforms can be expressed in HEK cells, there is no clear evidence of arrays forming in these cells, unlike in AQP-4 transfected Chinese hamster ovary (CHO) cells where square arrays have been demonstrated by freeze fracture
Because gap junctions are a known feature of urothelium and tight junctions are a feature of the apical-most cells, we used the technique of freeze fracture
to confirm the presence of these structures.
technique is a useful method to study the rubber particle cavitation of ABS (32).
The HDPE specimens were prepared by liquid nitrogen freeze fracture
to observe the surface of the ESC.
This is manifested by the fact that the freeze fracture
propagates through the dispersed XL-UHMWPE particles rather than along their interface.