Fluorescent in situ hybridization
assay using peptide nucleic acid probes for differentiation between tuberculous and nontuberculous mycobacterium species in smears of mycobacterium cultures.
Partial tetrasomy of chromosome 22 was confirmed by fluorescent in situ hybridization
Applications include use in in situ hybridization, fluorescent in situ hybridization
and other solid-phase assays where strong signal detection and sensitivity are desired.
The test utilizes next generation sequencing ("NGS") to analyze variants in the coding sequence of 315 genes and uses conventional Fluorescent in Situ Hybridization
("FISH") methods to identify large deletions, amplifications and translocations in 9 different chromosomal loci.
Examples of topics covered include the plasma cell labeling index, multicolor spectral karyotyping, detection of chromosome 13 deletions by fluorescent in situ hybridization
, identification and of clonotypic IgH VDJ sequences, real-time polymerase chain reaction of immunoglobulin rearrangements for quantitative evaluation of minimal residual disease, determination of telomerase activity and telomere length, clonality detection of the expanded t-cell populations in patients with multiple myeloma, and use of DNA micrrarray data.
Preimplantation genetic diagnosis using fluorescent in situ hybridization
has a positive predictive value of 83% and a negative predictive value of 81% when a panel of probes for five chromosomes is used, Catherine M.
Additional product lines built on this core technology include microarrays for genome-wide measurement of gene expression and for comparative genomic hybridization, as well as the SureFISH reagents, a highly specific and sensitive product line for oligonucleotide fluorescent in situ hybridization
A relatively new technique called comparative genomic hybridization may soon replace fluorescent in situ hybridization
as the method of choice for obtaining preimplantation genetic diagnoses for aneuploidy in blastomeres.
NYSE:A) today launched additional Agilent SureFISH probes, creating the largest offering of oligonucleotide-based fluorescent in situ hybridization
(FISH) assays on the market.