method and the two remaining HPLC methods (HPLC with fluorescence detection using either TCEP or TBP as a reducing agent and SBD-F as a labeling agent) showed no deviation compared with results of the GC-MS method.
2] administration, MTX concentrations measured by FPIA
were comparable to those obtained by LC-MS/MS (Fig.
digoxin assay is linear to a serum digoxin concentration of 5.
However, in Primavette samples Hcy obtained by FPIA
was approximately 11% higher than Hcy obtained by HPLC.
Amphetamines, THC, BEG, Assay [micro]g/L [micro]g/L [micro]g/L Syva Emit 700 (a) 35 (a) 60 (a) Beckman EIA 250 (b) 14 (b) 36 (b) Abbott FPIA
250 (a) 14 (b) 72 (a) SAMHSA cutoff 1000 50 300 Opiates, PCP, Assay [micro]g/L [micro]g/L Syva Emit 76 (b) 5 (b) Beckman EIA 76 (b) 5 (b) Abbott FPIA
76 (b) 10 (a) SAMHSA cutoff 2000 25 (a) Cutoff derived from point at which total CV exceeds 20%.
Sodium bicarbonate caused false positive with the EMIT opiate assay and PCP assay by FPIA
Adenosine analogs, such as 3-deazaadenosine, are effective but not compatible with assays based on S-adenosylhomocysteine hydrolase, including the FPIA
For example, the popular Abbott TDx FPIA
gave high results for phenytoin but low results for carbamazepine and valproate.
Stabilizing blood with 3-deazaadenosine interferes in the Abbott FPIA
assay for plasma homocyst(e)ine.
Negative results for all urine samples were obtained with the Abbott FPIA
, whereas the Mahsan-AM[P.
In surveys 2 and 3, tHcy was measured by the FPIA
method 7 of 23 and 6 of 17 times, respectively.