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a spotted or calico horse or pony

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3 Steroidogenesis of the ovary NOBOX 7q25 Early folliculogenesis FIGLA 2q12 Zona pellucida gene regulation FOXL2 3q23 Granulosa cell differentiation and follicle development SOHL1/2 13q13.
Mutation of FOXL2 in granulosa-cell tumors of the ovary.
Immunohistochemical Profile of Ovarian Sex Cord-Stromal Tumors Versus Ovarian Carcinomas Sertoli Ovarian AGCT Cell/SLCT Carcinomas [alpha]- + + - Inhibin Calretinin + + -/+ CK AE1/AE3 -/+ -/+ + (punctate pattern) CK7 - - + EMA - - + FOXL2 + + - SF1 + + - Abbreviations: AGCT, adult granulosa cell tumor; CK, cytokeratin; EMA, epithelial membrane antigen; FOXL2, forkhead box protein L2; SF1, steroidogenic factor 1; SLCT, Sertoli/Leydig cell tumor; +, positive; /, negative; -/+, focal or patchy positivity.
Like Yin and Yang, FOXL2 and SOX9 oppose each other's action to ensure together the establishment and maintenance of the different female and male supporting cell types respectively," Treier said.
196,197,199-203) Both adult and juvenile granulosa cell tumors express FOXL2 nuclear staining, with a sensitivity ranging between 75% (203) and 100%.
180) Adenocarcinomas are usually diffusely and strongly positive for EMA and CK7, and negative for inhibin, calretinin, and FOXL2.
Both juvenile granulosa cell tumor and steroid cell tumor usually express inhibin, calretinin, and FOXL2.
190,202,203,215,216) The use of FOXL2 is limited, since it is not expressed in approximately half of the cases of Sertoli-stromal tumors, particularly the retiform variant.
In this study, polymerase chain reaction (PCR) and direct sequencing were performed in 5 aGCTs from male patients to investigate whether FOXL2 402[right arrow]CrG (C134W) also occurs in granulosa cell tumors arising in men.
Both PCR and direct sequencing of the FOXL2 gene were performed as specified below.
The primers used for FOXL2 PCR were as follows: 5'-CCAGTACATCATCGCGAAGTTCCCG (forward) and 5'-CTCCGGCCCCGAAGAGCC (reverse).
The PCR products of FOXL2 (269 base pair [bp]) and [beta]-actin (198 bp) were separated on a 3% agarose gel and visualized by ethidium bromide staining.