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The spontaneous capability of sperm cells to bind exogenous DNA molecules and internalize them into nuclei can be exploited by using spermatozoa as vectors for delivering foreign genetic information to eggs during fertilization (Brackett et al.
The conclusion from this study parallels that from earlier work involving the injection of exogenous DNA into the circulation of laboratory animals, which showed rapid elimination kinetics (29, 30).
The expression of GFP beyond the stage of embryonic genome activation (4-cell stage in the pig) indicates that the exogenous DNA might have been integrated into the porcine genome.
The second approach uses an exogenous DNA sequence, called a competitor, that is almost identical to the target gene in size and sequence and is amplified with the same pair of primers used for the target DNA and consequently has the same behavior during PCR cycling (competitive PCR) (28).
DeKalb Genetics Corporation (DeKalb, IL) has patented a method for the production of stable, genetically transformed, Zea mays plants that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said plants.
An interesting analogy can be drawn between the present data and animal experiments documenting the transplacental transfer of exogenous DNA administered either intravenously or orally to pregnant animals (35, 36).
6,063,630 relates to a method of gene targeting in normal cells of vertebrate origin in which a fragment of exogenous DNA is introduced into the genome of the cells at pre-selected sites.
To control potential contamination of solutions and final probes with exogenous DNA, the experimental setup also included a control (25 mL of saline solution) that was carried through all subsequent procedures.
6,048,724 entitled, "Method of Producing Clonal Cell Strains which Express Exogenous DNA Encoding Glucagon-Like Peptide 1," and U.