has been formally validated by The European Centre for the Validation of Alternative Methods (ECVAM) and testing guidelines have been accepted by OECD for the testing of skin irritation (OECD 439) and skin corrosion (OECD 431).
Titled, "An In Vitro Skin Irritation Test (SIT) using the EpiDerm
Reconstructed Human Epidermal (RHE) Model", this peer-reviewed, PubMed-indexed video article is now available for online viewing on both the MatTek (WWW.
Evaluation of EpiDerm
Full Thickness-300 (EFT-300) as an In Vitro Model for Skin Irritation: Studies on Aliphatic Hydrocarbons" 101
The Updated EpiDerm
SIT is a modification of the previously validated EpiDerm
skin irritation method (ESAC Statement, April 2007).
org, noted that the "take home message" from these new studies is that EpiDerm
was not only successfully validated using just one endpoint (cytotoxicity -- MTT viability), but also that the EpiDerm
classification test results met the criteria set by the validated skin irritation test method.
17 /PRNewswire/ -- MatTek Corporation today announced that the ECVAM Scientific Advisory Committee (ESAC) endorsed the scientific validity of the Modified EpiDerm
Skin Irritation Test (SIT) at its November meeting.
, developed by Mat Tek is approved only for research use.
Regulates the capacity of water fixation by the epiderm
A thin layer of a lotion containing avocado sterols was applied to EpiDerm
and evaluated at 12, 24 and 48 hours after low dose UVB exposure to determine the levels of the skin's inflammatory response mediators including IL-1-alpha, PG[E.
Separately, MB Research Laboratories of Spinnerstown, Pennsylvania, conducted a non-animal ocular irritancy study using the Chorioallantoic membrane alternative to the Draize method, a dermal irritancy study using the MatTek EpiDerm
MTT viability assay, and an Acute Oral Toxicity test using the Up and Down procedure (UDP).