6) nmol RII produced/min x mg, respectively] and, as expected, 5 mmol/L EGTA
was a potent inhibitor of CN (3.
The only exception to these observations were the significantly greater shoot cadmium concentrations of plants grown in 500 mg Cd / Kg that were amended with EGTA
alone especially at 10 weeks after emergence.
Briefly, platelet suspension was lysed with equal volume of ice-cold Triton X-100 lysis buffer (2% Triton X-100, 10mM EGTA
and 100mM Tris-HCl, pH 7.
Soil bacterial populat ions were severely reduced by Cd, especially at the two highest concentrations (1,000 and 2,000 mg Cd/kg dry soil) in combination with the highest EGTA
2+]-free solution with 50 [micro]M EGTA
for 15 min before the application of PE (1 [micro]M) to induce the first transient contraction (con1).
Jerzy Barski, TV-Sat Magazine (Poland), Jacques Braun, Eurodata TV (France), Paolo Dalla Chiara, Pentastudio (Italy), Giovanna Maggioni, Upa (Italy), Asu Maro, Milliyet Sanat and Milliyet Daily (Turkey), Giacomo Mazzone, Eurovisioni (Europe), Mimi Turner, Hollywood Reporter (USA), Michel Gregoire, EGTA
(Europe), Jury chairman: Duilio Giammaria, Rai (Italy); technical expert Mauro Roffi, Mille Canali (Italy).
1-30 nM) in a final volume of 100 [micro]L binding buffer (10 mM Tris, ) mM EDTA, 1 mM EGTA
, 1 mM sodium vanadate(V), 0.
5,100 mmoUL NaCI, 1 mmol/L EGTA
,100 [micro]mol/L [Na.
25 M sucrose, 2 mM EGTA
, 200 ml distilled water, pH 7.
Wheat seeds were sown in various concentrations of Cd and EGTA
mixed with a growing medium composed of sieved soil and peat (2:1, v:v).
2],TEA-Cl, NMDG, CsCl, NaCl, KC1, HEPES; EGTA
, and K-aspartate were purchased from Sigma-Aldrich (St.
To quench extracellular calcium, 3 mM EGTA
was added to the erythrocyte suspension.
After incubation at 37 [degrees]C for 30 min, we inactivated the DNase by adding 1 [micro]L DNase Stop Solution (20 mmol/L EGTA
5, 150mM sodium chloride, 1% Triton, 1 mM EGTA
, 1 mM EDTA, 2.
4), 100 mM NaC1, 1 mM EDTA, 1 mM EGTA
, 1 mM sodium fluoride, 20 mM sodium diphosphate, 2 mM sodium orthovanadate, 1% Triton X-100, 10% glycerol, 0.