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This dsDNA construct was chosen because it exhibited superior detection and reproducibility of manufacture and a much lower degree of NSB to particle supports compared to a single-stranded construct.
Friedman and Carter also recommend consideration of ways in which the Guidance could be expanded to address oligos and benchtop synthesizers capable of making dsDNA.
The biological assays showed that dsDNA stock and dsDNA:TOC ratio were similar in organic and chemical fertiliser sites (Table 2).
The relative amount of dsDNA after cDNA synthesis was inversely related to the difference in probe variance between samples (Fig.
3] Nonstandard abbreviations: ANA, antinuclear antibody; CTD, connective tissue disease; SLE, systemic lupus erythematosus; IF-ANA, immunofluorescence-based method for antinuclear antibodies; EIA, enzyme immunoassay; COBAS-ANA, COBAS Core HEp2 ANA ElA; MBL, Medical & Biological Laboratories; dsDNA, double-stranded DNA; SS, Sjagren syndrome; SSc, systemic sclerosis; DM/PM, dermatomyositis and polymyositis; MCTD, mixed connective tissue disease; RA, rheumatoid arthritis; 95% CI, 95% confidence interval; and AUC, area under the curve.
A completely closed-tube method using a generic dsDNA dye for effective genotyping and scanning has been elusive.
For example, a good percentage (42%) of SLE sera had autoantibodies against dsDNA as well as against SSA/52 (54%), SSA/60 (48%), and ssDNA (49%).
1] Nonstandard abbreviations: TPMT, thiopurine S-methyltransferase; ASA PCR, allele-specific amplification PCR; SNP, single-nucleotide polymorphism; and dsDNA, double-stranded DNA.
Pre-programmed Applications : dsDNA, ssDNA, RNA, and Oligonucleotides, Protein UV (A280), Protein Dye, Bradford, Lowry, BCA, Biuret, Cell density
Serial 10-fold dilutions of calf intestinal AP (New England Biolabs) were prepared and incubated with the phosphorylated dsDNA substrate (final concentration in the reaction of 1 ng/[micro]L) for 1 h at 37[degrees]C in Buffer 3 (from the enzyme supplier).
Evaluation of a new automated enzyme fluoroimmunoassay using recombinant plasmid dsDNA for the detection of anti-dsDNA antibodies in SLE.
These new data appear to show that the 300 mg and 900 mg doses of Riquent reduce antibodies to dsDNA more than the previously studied 100 mg dose.
7] Nonstandard abbreviations: ANA, antinuclear antibody; SLE, systemic lupus erythematosus; MCTD, mixed connective tissue disease; SS, Sjogren syndrome; IFA, immunofluorescent analysis; EIA, enzyme immunoassay; and dsDNA, double-stranded DNA.
The dsDNA is denatured to single-stranded DNA (ssDNA) by breaking the hydrogen bonds between the complementary base pairs adenine-thymine (A-T) and guanine-cytosine (G-C).
The specific competitor decreased the amount of dsDNA template (or increased the Ct value) in the presence of NF-[kappa]B (Fig.