Daejon, Korea) for 72 h until they recovered from the operation of dorsal aorta cannulation.
A 50 cm long PE 50 tubing (Clay Adams PE 50 tubing, Parsippany, NJ, USA) with a bubble of about 5 to 6 cm on one end was used for the dorsal aorta cannula after it was flushed with the heparinized Cortland saline solution (Houston, 1990).
Embryologically, the carotid arteries originate in the third aortic arch and the dorsal aorta
As a part of our studies on essential amino acid requirements of rainbow trout, the purpose of the present study was to estimate the dietary methionine requirement based on the plasma free methionine and ammonia concentrations in dorsal aorta cannulated rainbow trout.
Daejon, Korea) for 72 h until the fish recovered from the operation of dorsal aorta cannulation.