Keywords: Ilex paraguariensis Glycemi Insulin Diabetes Glycation Disaccharidase
For determination of the disaccharidase activity 50 [micro]l of homogenate were pre-incubated at 37 [degrees]C for 5 min, in the absence (control) or in the presence of the Et0Ac or n-BuOH fractions of paraguariensis (treated).
Table 5A and B shows the acute effect of the Et0Ac and n-BuOH fractions on in vitro disaccharidase activity.
The ability of the Et0Ac and n-BuOH fractions to inhibit in vitro disaccharidase activity was observed as a very short-term effect after duodenum incubation.
Activities of mucosal disaccharidases including lactase, sucrase, and maltase have long been used as indicators of mucosa maturation and gut digestive function in pigs (Hampson and Kidder, 1986).
Disaccharidase activity was assayed according to the method of Hou et al.
The data for disaccharidase activities are presented in Table 2.
Dahlqvist (1961) reported that the small intestine had powerful disaccharidases activities which showed different locations along the small intestine; the activities of sucrase and maltase were mainly localized in the distal part of the small intestine, and lactase was localized in the proximal part.
Properties of gastrointestinal disaccharidase in pig.
The jejunal and ileal mucosa disaccharidase
activities were determined as described by Petersen et al.
However, Thomsen and Tasman-Jones (1982) observed decreased disaccharidase activities in the intestinal mucosa by dietary fibre.
Disaccharidase levels of the rat jejunum are altered by dietary fibre.