Plasma P4 concentrations were measured using an enzyme immunoassay kit after diethylether
The solvent was removed in vacuum and the residue was extracted with diethylether
stimulated hydrolytic rate of long chain PC ([C.
First, free CTs were extracted with a mixture of acetone, water and diethylether
The phospholipids, diacylglycerol, FFA, TG, and CE were separated by use of n-hexane and diethylether
, with acetic acid as a solvent.
5 ml for flower and fruits) was dissolved in diethylether
(lml) and collected and dehydrated by anhydrous sodium sulfate.
The analysis procedures were carried out as follows: five grams of the deposit under investigation were accurately weighed and the solid material was extracted from the sample by diluting it several times in petroleum ether and diethylether
Several extraction solvents were attempted: diethylether
, ethylacetate, chloroform, n-hexane, n-pentane, and dichloromethane.
Samples (2 mL) were extracted with 2 mL diethylether
during 30 min.
The reaction was terminated with 2 mL of saturated NaCl and the benzoylpolyamines extracted in 2 mL of diethylether
(anhydrous, HPLC grade).
The ester, 2, was extracted by 30 mL diethylether
and collected after distillating the solvent.
On day 31, mice were killed with an overdose of diethylether
, and the liver, lung, spleen, thymus, and tumors were quickly removed and weighed.
Solvent P1 P2 P3 P4 P5 DMF ++ ++ ++ ++ ++ DMSO ++ ++ ++ ++ ++ 1,4-Dioxane ++ ++ ++ ++ ++ n-Hexane -- -- -- -- -- Dichloromethane ++ ++ ++ ++ ++ Diethylether
-- -- -- -- -- Methanol -- -- -- -- -- THF ++ ++ ++ ++ ++ EMK ++ ++ ++ +- +- Acetonitrile -- -+ -+ -- -- Ethanol -- -- -- -- -- P1, poly(VBOPE); P2, oxime polymer; P3, TSC polymer; P4, hydrazone polymers; P5, sulfonic acid hydrazone polymer; ++, soluble at room temperature; +-, swelling or party soluble at room temperature; -+, swelling or party soluble by heating; -, insoluble.
The products were filtered hot, washed several times with hot EtOH followed by diethylether
and finally dried in a vacuum desiccator over anhydrous Ca[Cl.
We measured testosterone after performing diethylether
extraction with an in-house RIA using a polyclonal antitestosterone-antibody (AZG 3290; a gift from Dr.