3 mmol/L of each deoxynucleotide
, 1 U JumpStart Taq polymerase (all Sigma-Aldrich), 0.
Nonspecific DNA repair enzymes excise damaged DNA lesions to release deoxynucleotides
40 microelectronic chip (a) Supplies common to both genotyping methods included Thermowell 96-well plates, DNA polymerase, deoxynucleotides
, primers, Thermowell sealers, tips, and 96-microwell plates.
The complete electropherograms of the intracellular nucleotides, deoxynucleotides
, nucleosides, and bases in RBCs from healthy individuals and ADA/SCID patients are reported in Fig.
These differ from the results (C < G < A < T) based on oligonucleotides with 3' deoxynucleotides
4x]Mix, we used FastStart[R] Taq DNA polymerase (5 U/[micro]L), PCR-grade deoxynucleotides
4, 50 mmol/L KCl), 200 [micro]mol/L deoxynucleotides
(Amersham Pharmacia Biotech), and 3 mmol/L [MgCl.
5 mmol/L MgCIZ, 1 mL/L Triton X-100, 200 [micro]mol/L each of the four deoxynucleotides
, 1 [micro]mol/L each of the primers, and 3 U of Dynazyme (Finnzymes) in a final volume of 50 [micro]L.