The method uses a thermophilic RNase H2 enzyme from Pyrococcus abyssi, which functions optimally in conditions compatible with thermophilic DNA polymerases
, such as Taq polymerase.
The expression vector with Taq DNA polymerase
gene, developed in the present study, will certainly expand more avenues for heat-tolerant genetic transformation in plants.
Therefore, it is important to understand the true prevalence of HBV DNA polymerase
mutation analysis among treatmentnaive patients with CHB in different areas of China, because most of them will be treated with NAs at first.
4] and DMSO for each DNA polymerase
was calculated separately according to the recommendation of the producer.
Phospho-linked nucelotides allow observation of the immobilized complex as the DNA polymerase
produces a completely natural DNA strand.
Reactions using KAPA2G Fast DNA polymerase
substituted 1X KAPA2G Buffer A instead of 50 mmol/L Tris (pH 8.
The product of the gene, Taq DNA polymerase
was also isolated.
My colleagues and I generated a mouse strain that is mutated in the Polk gene (which encodes DNA polymerase
K) and have sought to determine the 'cognitive' substrate for this enzyme.
herbaceum) of A genome, were found to be free of virus when screened by PCR and (Phi) 29 DNA polymerase
The Thermo Scientific Phusion Green High-Fidelity DNA Polymerase
and Luminaris Color qPCR master mixes use proprietary tracking dyes to speed up the pipetting process.
9,10) After an oligonucleotide is annealed to the template strand of DNA to be sequenced, a DNA polymerase
synthesizes DNA by extending the 3' end of the nascent strand using the information encoded in the template strand.
The medicine is an upgraded version of the Phi 29 DNA polymerase
utilised in isothermal amplification, which provides improved properties.
The nucleoside triphosphates of pyrimidine analogs (2-aminopyridin-5-yl and 3-methyl-2-pyridon-5-yl), which lack the 2-keto group of C and T, strongly inhibited PCR amplification by Taq DNA polymerase
or the Klenow fragment of Escherichia coli DNA polymerase
The enzyme, known as Rad18, detects a protein called DNA polymerase
eta (Pol eta) and accompanies it to the sites of sunlight-induced DNA damage, enabling accurate repair.
4 [micro]1 Taq DNA polymerase
(5 units) (GenetBio Co.