The Hershberger assay is one of the assays selected for validation based on the need for in vivo screening to detect androgen agonists or antagonists by measuring the response of five sex accessory organs and tissues of castrated juvenile male rats: the ventral prostate, the seminal vesicles with coagulating glands, the levator ani and bulbocavernosus muscle complex, the Cowper's glands, and the glans penis.
1932, 1933a, 1933b), the Cowper's glands (Wainman and Shipounoff 1941), and the glans penis (Bulbring and Burn 1935; Dingemanse et al.
Similar to Warnnissorn and colleagues, (8) we found that the perimeatal region was involved and the absence of sweat, Littre or Cowper's glands
was compatible with the hypothesis that MC is a bimodal cancer.
The following tissues were removed and weighed at necropsy: liver and combined kidneys (to 1 mg), Cowper's glands, levator ani/bulbo cavernosus muscles (LA/BC), seminal vesicles (with coagulating gland), ventral prostate, glans penis, and combined adrenal glands (all to 0.
In all of the experiments, a dose of 5,000 [micro]g/kg/day FIN significantly inhibited the growth of the seminal vesicles, ventral prostate, Cowper's glands, and glans penis compared with animals administered only TP (Table 1).