After preparation of the background wells and positive control wells, the sample wells were prepared: 100 [micro]L of final Assay Buffer, 50 [micro]L of GPx Cosubstrate
Mixture, and 20 [micro]L of sample (plasma) were added.
Activity was evaluated using GSH as the cosubstrate
(Paglia et al.
However, the 3HV fraction in the copolymer has been relatively low because the propionic acid, a cosubstrate
for the 3HV units, can also be assimilated for cell growth of R.
The GSH indirectly support the detoxification of free radical components by its function as cosubstrate
of the antioxidant enzymes such as glutathione peroxidases (GPxs) (Regoli & Principato 1995).
Methylenetetrahydrofolate reductase (MTHFR) is the enzyme that catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate
for homocysteine remethylation to methionine (7,8).
These transfer electrons directly to the electrode thereby eliminating the need for the reduction of an oxygen cosubstrate
putida could not metabolize 1,5-dimethylnaphthalene even when naphthalene was used as a cosubstrate
Localized production of hydrogen peroxide by the root tip of the, as yet, free-living striga provides the rate limiting cosubstrate
for host cell wall peroxidases.