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  • noun

Words related to Kumasi

a city in southern Ghana

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BSA concentrations were obtained from the Bradford method relying on the binding of the dye Coomassie Blue G250 to protein [32], The absorbance at 595 nm was determined by a UV-vis spectrophotometer (UV-1601, Shimadzu, Japan).
The target band in SDS-PAGE was collected, destained with Coomassie brilliant blue in 60% acetonitrile (ACN), reduced at 37[degrees]C by 1 M dithiothreitol (DTT) for 0.
After 2D electrophoresis, the gels were fixed with 10% methanol/7% acetic acid and stained with colloidal Coomassie.
The data shown here demonstrate that salicylic acid and cold shock induces the expression of some proteins in large scale because they are detected by Coomassie Blue; these proteins might be involved in cell protection in a similar manner as the HSPs from several families.
Coomassie blue stain Gel stain Gel electrophoresis buffer Liquid to conduct charge in the chambers, stable pH Mini/microcentrifuge 8,000 or 15,000 x g SDS-PAGE chamber with appropriate power supply Precast gels Use Tris-HCl 12% gel that meet chamber specifications Micropipettes of varying sizes with appropriate tips Microcentrifuge tubes Hot water bath (95[degrees]C)
025% Coomassie Brilliant Blue R (Sigma Chemical Company, St Louis, USA) and once de-stained were dried onto cellophane for analysis by densitometry.
The white light side has three 8-watt fluorescent tubes that allow researchers to look at Coomassie blue-stained protein gels, methylene blue-stained DNA gels and autoradiograms.
Proteins were separated in second dimension on 12 per cent SDS-polyacrylamide gels (15) in a vertical electrophoretic unit PROTEAN II XI (BIO-RAD, Hercules, CA, USA) at a constant voltage of 250 V for 5-6 h at 100 W and gels were stained with coomassie brilliant blue R250 to visualize proteins.
An improved method for protein measurement may be Bradford's method, which measures the shift in absorbance of the dye Coomassie when it binds to protein.
e permaneceram em solucao contendo 2% Coomassie Blue R250 ate aparecerem as bandas de proteina.
We assessed the purity of the Hpx-purified solution by separation on 10% SDS-PAGE according to Laemmli (16), followed by Coomassie stain.
After electrophoresis the gel was stained with Coomassie Brilliant Blue R 250 according to standard procedures.
Coomassie Brilliant blue G250,Ortho Phosphoric acid (Purity 85%, M.
Further back on the bottom line is Expert Champion, littersister to the 1974 Derby ace Jimsun, and it traces eventually to Coomassie, the dual Waterloo Cup winner of 1877 and 1878, whose pedigree was never established.
7) Proteins resolved in a 2-DE gel can be stained with Coomassie brilliant blue, silver or fluorescent dyes.