Prenatal genetic testing has made significant progress since its early introduction in the 1970s when fetal chromosome analysis became feasible with the development of amniocentesis and the ability to culture fetal cells present in the amniotic fluid sample.
After the considers above, the essential question is how to make sure the inherited traits from the fibroblasts are stable, the healthy experimental animal should be chose, the viability of the isolated cells should be assessed before freezing and after recovery, the chromosome analysis could illustrate the cells are normal or malignant and show if the chromosome structure is stable (Li et al.
Chromosome and fluorescence in situ hybridization (FISH) studies are typically performed on peripheral blood or bone marrow samples and require dissociated cells for interphase FISH and viable cells capable of division to generate metaphases for chromosome analysis.
Using a reorganized structure, this edition has been updated to include the latest information on the Human Genome Project, identification of new classes of RNA molecules, new molecular genetic and chromosome analysis techniques, further development of preimplantation genetic diagnosis, improved methods for antenatal and neonatal screening, self-assessment questions, and case study scenarios.
As tier 1 testing in such children, I usually order a microarray-based comparative genomic hybridization (array CGH), a test that has largely replaced high-resolution chromosome analysis in this population.