Silybin (1) and its derivatives (2-4) inhibited the NADPH oxidase activity in PMA-stimulated cell lysate
by a concentration dependent manner (Fig.
A positive signal was detected when PCR-amplified SiHa and HeLa cell lysate
dilutions, plasmids, and reference HPV-positive samples were hybridized with the specific oligoprobes.
PCR with 4 [micro]L of cell lysate
rather than 1 [micro]L only marginally affected the yield of PCR products, whereas 35 cycles instead of 30 cycles increased the amount of PCR products (Fig.
The serum as well as the cell lysate
2-color ULS(TM) protein labeling and fluorescent detection kits have been validated on all currently available antibody array platforms and will have their market launch at the upcoming AMT Meeting in London, October 11-13.
We recently developed an oligo(dT)-coated manifold support that allows mRNA to be isolated directly from cell lysates
by hybridization followed by transfer through the different washing and enzymatic steps of the assay with minimal pipetting (13).
Since 2004, Aushon has recognized the power of reverse phase arrays and tried to enable its widespread use via a novel solid-pin arrayer architecture that overcomes the difficulties of arraying highly viscous samples like cell lysates
Direct assay of cell lysate
requires the normalization of amounts of DNA, which was achieved through calculating the rlu ratio of telomere repeats to Alu sequence.
Nearly 9,000 of products have been added in this launch such as Recombinant Human ZNF80 293 Cell Lysate
, Recombinant Human VAMP8 293 Cell Lysate
, Recombinant Human YIF1B 293 Cell Lysate
, and Recombinant Human BBS5 293 Cell Lysate
Now, with the Total Protein Master Kit, the benefits of the Simple Western are available to researchers who are currently forced to run a gel and deal with tedious staining protocols just to visualize and quantitate an overexpressed protein or evaluate the amount of a protein in a complex cell lysate
After 5 minutes, the solubilized cell lysate
was removed and vortexed.
No kinetics constants have, to our knowledge, been previously published for a CN activity assay by use of a Jurkat cell lysate
In cell culture studies, apigenin treatment resulted in cell growth inhibition and induction of apoptosis, which correlated with increased accumulation of IGFBP-3 in culture medium and cell lysate
In the in vitro assay, SK-N-SH cell lysate
was incubated with TCDD ([10.
Western blotting: Recombinant HPV-16 L1 (rL1) expression profile from the crude cell lysate
of the yeast clones was obtained by immtmoblot.
Uninfected BSC40 cell lysate
was treated similarly to the infected cells described above.