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5% NaCl -ve -ve -ve -ve -ve -ve -ve Gas Production Glucose +ve +ve +ve +ve +ve +ve +ve Sucrose +ve +ve +ve +ve +ve +ve +ve Trehalose +ve +ve +ve +ve +ve +ve +ve Mannitol +ve +ve +ve +ve +ve +ve +ve Maltotriose +ve +ve +ve +ve +ve +ve +ve Fructose +ve +ve +ve +ve +ve +ve +ve Raffinose -ve -ve -ve -ve -ve -ve -ve Gram stain test +ve +ve +ve +ve +ve +ve +ve Catalase test -ve -ve -ve -ve -ve -ve -ve KOH test -ve -ve -ve -ve -ve -ve -ve +ve: positive, -ve: negative, A1, A2, A3, A4, A5, A6 and A7: strains from A.
Generally catalase test is used to differentiate Streptococcus from Staphylococcus.
What two main bacterial groups of gram-positive cocci does the catalase test distinguish between?
Although other bacteria were more difficult to identify, some other methods used to aid in classifying each bacterial sample were performed, including the oxidase test, catalase test, and stab cultures.
Last, perform a catalase test (if the organism is not growing on MAC or EMB), or a commercial identification kit system (if the organism is growing on MAC or EMB).
After incubation, the organism is identified as an enterococcus on the basis of morphology of cultures and a negative catalase test and if esculin hydrolysis and PYR reaction (pyrrolidonyl-[beta]-naphthylamide hydrolysis) are positive.
A motility test (motility agar, Difco) and catalase test were also conducted in microtiter plates.
by a 2-week culture on MacConkey agar and a heat catalase test but weakly positive by 3-day arylsulfatase and 2-week catalase testing (8).