Swords of cell death: caspase
activation and regulation.
The new method of killing cancer cells - called Caspase
Independent Cell Death (CICD) - led to the complete eradication of tumours in experimental models.
Apoptotic protein expression and activation of caspases
is changed following cholinergic denervation and hippocampal sympathetic in growth in rat hippocampus.
The principle of this method is based on staining of chromogen DAB which will bind with the antibody of p53, Bcl-2, Caspases
8 and 9, and formed brown color on the cell membrane.
The inflammasome: A molecular platform triggering activation of inflammatory caspases
and processing of proIL-beta.
Bcl-2 is the group of proteins which together with caspases
plays an important part in modulating apoptosis .
One of these cleaved caspases
is present on the activated caspase
-3, a ubiquitously distributed caspase
which is the main effector caspase
of the apoptotic cascade within cells [24,27].
This is through the release of Cytochrome c from mitochondria is a central event in the death receptor-independent, "intrinsic," apoptotic pathway which facilitates activation by Caspase
9 of the effector Caspases
, which subsequently activates Caspase
3, and ultimately leads to the apoptotic cell death.
Meanwhile, survivin specifically inhibits the induction of apoptosis and activates caspases
3, 7 and 9 (Cheung et al.
However, up till now, caspases
have never been explored as a suitable target to modulate apoptotic cell death in atherosclerosis.
While Adriamycin was antagonistic toward heart cells, resveratrol and quercetin decreased the activity of caspases
(protein- degrading enzymes) involved in apoptosis (programmed cell death) in these cells while not interfering with Adriamycin's caspase
activity in cancerous cells.
To evaluate the ability of caspases
in induction of apoptosis by O.
However, activation of caspases
can also promote a variety of vital cellular processes.
Scientists have determined that this enzyme, called caspase
-11 in mice--the parallel enzyme in humans is a combination of caspases
4 and 5--enables components in immune cells to fuse and degrade the bacteria that cause Legionnaires' disease, a type of pneumonia.
Measured responses included activation of ERK and JNK, mitogen-activated protein kinases involved in cell growth, proliferation, and apoptosis (programmed cell death); activation of caspases
8 and 9, enzymes that also are involved in apoptosis; and release of prolactin, a hormone that helps regulate hundreds of biological functions, including metabolism, reproduction, and lactation.