Multiplex PCR was used as a sensitive technique to identify C.
Therefore, the aim of the work is to assess the value of using biotechnology in rapid detection of C.
In the present study, toxin antitoxin neutralization test on the skin of albino guinea pigs and lethal toxicity test I/P in mice were applied using specific antitoxin to detect and identify the toxigenic strains of C.
The highest dilution yielding an amplicon with 3 bands (426 bp of C.
Augustynowicz, Faculty, Department of Sera and Vaccine Evaluation, National Institute of Hygiene, Warsaw, Poland, for providing us the reference strains of C.