In Figure 1 and 2, the effect of sucrose preculture concentration on viability of Brassia rex PLBs is presented.
Effects of Duration Exposure to DMSO Treatments at 24[degrees]C and 0[degrees]C on Brassia Rex Plbs Viability prior to cryopreserved (+LN) and non cryopreserved (-LN) PLBs:
For the TTC test, Figure 5 and 6 showed the viability of Brassia rex PLBs after exposure to variety length of DMSO treatment at two different temperature (24[degrees]C and 0[degrees]C), with (+LN) and without (-LN) their subsequent storage in LN.
For the overall of Part II experiments, using TTC test, it can be concluded that size type 2 Brassia rex PLBs which was underwent 10 min DMSO treatment maintain at 0[degrees]C have the best viability (Fig.
50 M sucrose concentration gave the highest survival rate of Brassia rex PLBs.
It is appeared that 10 min of exposure to DMSO treatment was superior to 0, 20 and 30 min times of exposure since there is high viability of Brassia rex PLBs tested by TTC.
In the present study by using the TTC assay, the results show that at 0[degrees]C exposure temperature to DMSO treatment given highest viability of Brassia rex PLBs compared to 24[degrees]C of exposure for both cryopreserved (+LN) and non cryopreserved (-LN) PLBs.
From the experiments that have been conducted, the highest amount of chlorophyll content being detected derived from non cryopreserved Brassia rex PLBs which is underwent 10 min DMSO treatment at 0[degrees]C.