Similar values for the binding constants
were estimated, with some flavonoids showing a slightly better affinity for the whole protein respect to the isolated domain.
were measured in 10 mM buffer with 5 or 10 mM Ca[Cl.
From the temperature dependence of the binding constant
, it is possible to estimate the values for the thermodynamic functions involved in the binding process.
eG and eH-G represent the absorption coefficient for the sample and the sample-DNA complex respectively and K is binding constant
were K is the binding constant
, i and io are the peak currents with and without DNA and A is the proportionality constant.
(M-1), free binding energy (kJ mol-1) and mode of interaction with the double helical structure of DNA.
Ni and Ki are number of binding sites, and binding constant
of the i class of binding site, CL,0 and CP,0 are the original concentration of zinc ion and HSA .
, partition coefficient between bulk and miceller phase, and the number of drug molecules incorporated per micelle were calculated.
We also determined the binding constant
of glucose to the neutral boronic acid derivatives.
Based upon the decrease in peak current of NPF by the addition of different concentration of DNA, ranging from 20 uM to 40 uM, the binding constant
was calculated according to the equation 2 .
The change in peak potential and peak current was used to probe the mode of interaction, binding constant
and free binding energy.
Where bT is the Temkin constant associated with sorption enthalpy (kJ mol-1), R is the gas constant (kJ mol-1 K-1), AT is the Temkin equilibrium binding constant
(L g-1), and T the absolute temperature (K).
i] are the binding constant
and the number of binding sites for the ith class of binding sites, respectively.
In fact, the binding constants
(K) were obtained for some porphyrin-metal ion complexes (32).
During the experiment, it has been observed that very high metal ion concentrations and high metal-ligand binding constants
serve to reduce the rate of aggregation.