Key words: albumin, bromcresol green, protein electrophoresis, avian, penguin, Spheniscus species
Albumin is the most abundant protein in normal plasma and is integral to the maintenance of physiological balance.
It was considered that modified albumins might change response curves owing to altered affinity for antibodies or loss of reactivity of subsets of reactive epitopes.
Serum albumins from different animal species were examined as another model for structural modification of albumin (Table 2).
The safety of one raw material, Bovine Serum Albumin (BSA) has become most critical since it is a widely used ingredient in the manufacture of veterinary vaccines, as well as in research, diagnostics, pharmaceuticals, and biotechnology.
According to Michael Budnick, Manager, Technical/Marketing Services, "We believe Bovuminar BSA is the first commercially available bovine albumin to be produced by a virus validated process.
Further complicating the search for biomarkers is the high abundance of 22 proteins, including albumin and immunoglobulins, that comprise approximately 99% of the protein mass in serum (3-5).
Chicken IgY antibodies may be superior to other immunoaffinity-based albumin depletion methods because of their high affinity and specificity for albumin from multiple mammalian species (19), so we used them in this experiment.
The similar physicochemical characteristics of CA and human albumin presumably allowed reproducible and associated responses, and the 22-kD difference in molecular weight enabled these 2 albumins to resolve easily, which made CA an ideal internal calibrator.
These nonalbumin proteins were resolved from the albumins.
Bisalbuminemia (or alloalbuminemia) is characterized by the presence of two albumin
components (in equal or unequal amounts) on serum protein electrophoresis (1).
The site included complete citations and summaries of recent publications about albumin
By virtue of its concentration, albumin
regulates plasma osmotic pressure; however, it functions primarily as a transport protein and reversibly binds a wide array of ligands, such as fatty acids, steroids, bilirubin, tryptophan, and copper (1, 3).
Congenital analbuminemia is a rare recessive inherited disorder characterized by an absence or very low concentrations of serum albumin
The author starts with a concise historical overview and a detailed illustration of the structural information and chemical properties of albumin
from human and bovine sources.